Download Advances in Microbial Physiology, Vol. 10 by A.H. Rose (ed.), D.W. Tempest (ed.) PDF

By A.H. Rose (ed.), D.W. Tempest (ed.)

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Place in the thymine-deprived organisms. After 12 to 24 h, the cultures contained numerous minute particles, thin, flexible, threadlike filaments and other minute, often bizarre, forms like the minicells of E . coli, devoid of DNA. Although the effects of inhibition of protein and RNA synthesis on the synthesis of the other macromolecules have not yet been demonstrated, it may be tentatively concluded that synthesis of lipids, proteins and DNA in the mycoplasma strain Y are not stringently co-ordinated, and that a t least this mycoplasma has no stringent control mechanisms for co-ordinating macromolecular synthesis.

Arginini and M . , 1972 and unpublished data). Although with the slowly growing mycoplasmas it is difficult to determine the correct harvesting time, attempts to obtain membranes from M . , 1970). It is not yet clear how the wall-less mycoplasmas resist osmotic lysis during most of their growth cycle, and why it is least effective in the cold. One explanation is that, because of the high surface to volume ratio of the minute cells, rapid liberation of the internal solutes may occur upon transfer to a hypotonic solution, quickly lowering the internal osmotic pressure so as to cushion the osmotic shock.

1970) was, however, found to have different properties, this identification is untenable. Shaw et ul. (1970) have accordingly proposed an alternative structure, namely glycerylphosphoryldiglucosyl diglyceride. The tentative structure of this phosphoglycolipid is : CH,OH I CHOH I OH I CHZ-0-P-OH l OH I CH,-O-CO-I% Since this phospholipid is completely resistant to hydrolysis by phospholipases A, C, and D, the generic name “phosphatidyl” is inapplicable and phosphoglycolipid seems to be more appropriate.

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